External
Biochemistry Experiment Manual
- Author
- 吳繡賢 李惠春
- Publisher
- Tzu Chi University 慈濟大學
- Source
- LibreTexts
- License
- CC BY-NC-SA
- Language
- EN
- Category
- Basic Sciences
Contents· 17 sections
- 01: Gravimetric Calibration of Micropipettes
- 02: Preparation of Ovalbumin and Lysozyme
- 2.01: Protein Fractionation
- 2.02: Protein Quantification
- 2.03: Gel Filtration Chromatography
- 2.04: Ion-exchange Chromatography
- 03: Gel Electrophoresis of Protein
- 04: Kinetic Characteristics of Acid Phosphatase
- 05: Glucose Tolerance Test and Determination of Blood Glucose
- 06: Quantitative Determination of Serum
- 07: Recombinant DNA
- 7.01: Plasmid DNA Preparation and Nucleic Acid Quantitation
- 7.02: Restriction Enzyme Digestion of Plasmid DNA and Agarose Gel Electrophoresis
- 7.03: Gene Amplification by Polymerase Chain Reaction (PCR)
- 08: Ligation of a restriction enzyme digested plasmid DNA
- 09: Transformation of ligated plasmid DNA
- 10: Green Fluorescence Protein (GFP) Purification