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IVC Biol 15: Microbiology Lab Manual

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IVC Biol 15: Microbiology Lab Manual

This is a Microbiology lab manual that goes with a separate textbook for IVC students for a 5 unit class. This lab manual provides students with fundamental skills and concepts of the structure and physiology of non-disease and disease producing microorganisms with particular attention to bacteria. Basic techniques for culturing, staining, counting and identifying microorganisms. Designed to meet the requirement to enter one of the medical fields as well as general education.

Biology
Author
Emalee MacKenzie
Publisher
Irvine Valley College
Source
LibreTexts
License
CC BY-NC-SA
Language
EN
Category
Basic Sciences
Read online Download PDF (open licence)
Contents· 51 sections
  1. 01: INTRODUCTION. SAFETY AND AGAR PLATES
  2. 1.01: Laboratory Safety Rules
  3. 1.02: Required Laboratory Supplies (Student Responsibility)
  4. 1.03: USING AGAR MEDIA
  5. 02: EXPOSURE PLATES
  6. 03: DECONTAMINATION
  7. 04: MICROSCOPY
  8. 05: MEASURING WITH A MICROSCOPE
  9. 06: STERILE TECHNIQUE
  10. 07: SMEAR AND SIMPLE STAIN
  11. 08: GRAM STAIN
  12. 09: ISOLATION BY STREAK PLATE
  13. 10: BACTERIAL GROWTH CURVE
  14. 11: EPIDEMIC
  15. 12: OUTBREAK
  16. 13: SURVEY OF FUNGI
  17. 14: ALGAE
  18. 15: PROTOZOA
  19. 16: TEMPERATURE EFFECTS
  20. 17: ANTIBIOTICS
  21. 18: ANTISEPTICS AND DISINFECTANTS
  22. 19: ALCOHOL FERMENTATION
  23. 20: SKIN AND UPPER RESPIRATORY BACTERIA
  24. 21: ANTIBIOTIC PRODUCING MICROBES IN SOIL
  25. 22: VIRAL PLAQUE ASSAY
  26. 23: ACID FAST STAIN
  27. 24: CAPSULE STAIN
  28. 25: BACTERIAL COUNTS ON FOOD
  29. 26: AEROBIC VS ANAEROBIC GROWTH
  30. 27: ENDOSPORE STAIN
  31. 28: EFFECTS OF UV LIGHT ON BACTERIA
  32. 29: Probiotics
  33. 30: BLOOD TYPING
  34. 31: TESTING YOUR KITCHEN
  35. 32: METABOLIC TESTING
  36. 32.01: Starch Hydrolysis Test
  37. 32.02: Skim Milk Test
  38. 32.03: Spirit Blue Agar Test
  39. 32.04: Phenol Red Fermentation Test
  40. 32.05: Methyl Red Voges-Proskauer (MR VP) Test
  41. 32.06: Nitrate Reduction Test
  42. 32.07: Kligler Iron Agar (KIA) Test
  43. 32.08: Mannitol Salt Agar (MSA) Test
  44. 32.09: Eosin Methylene Blue (EMB) Test
  45. 32.10: Indole Broth Test
  46. 32.11: Urease Test
  47. 32.12: Simmons Citrate Agar Test
  48. 32.13: Motility Test
  49. 32.14: Oxidase
  50. 32.15: Catalase Test
  51. 32.16: Flow Chart