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Lab Manual: Synthetic Biology Protocols
The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions.
- Author
- Nathan Reyna, Ruth Plymale, & Kristine Johnson
- Publisher
- Ouachita Babtist University & University of New Hampshire
- Source
- LibreTexts
- Language
- EN
- Category
- Basic Sciences
Contents· 30 sections
- 01: Protocols
- 1.01: Visual Representation of the Protocol
- 1.02: Plasmid DNA Extraction (Mini-Prep)
- 1.03: DNA Double Digestion
- 1.04: Gel Loading and Running
- 1.05: Ligation of Products
- 1.06: Cell Transformation
- 1.07: Inoculation
- 02: Appendices
- 2.01: Appendix I- Antibiotics and Color Coding
- 2.02: Appendix II- Using the Denovix
- 2.03: Appendix III- Using the Nanodrop
- 2.04: Appendix IV- Using the Qubit
- 2.05: Appendix V- Common Agarose Gel Questions and Tips
- 2.06: Appendix IV- Preparing Glycerol Stocks
- 03: Ancillary Materials
- 3.01: Instructors Notes
- 3.1.01: Making Media (LB Broth and Agar)
- 3.1.02: Adding Antibiotics
- 3.1.03: Linearized Backbone PCR Protocol
- 3.1.04: DNA Extraction Supplies
- 3.1.05: Double Digestion Supplies
- 3.1.06: Gel Loading Running Supplies
- 3.1.07: DNA Ligation Supplies
- 3.1.08: Transformation Supplies
- 3.1.09: Inoculation Supplies
- 3.1.10: Glycerol Stock Supplies
- 3.1.11: Linearizing Backbone Supplies
- 3.1.12: Tips for Organizing the Lab for Large Classes
- Before you begin a semester overview