External
Supplemental Modules (Biochemistry)
- Author
- Michael Blaber
- Publisher
- Florida State University
- Source
- LibreTexts
- Language
- EN
- Category
- Basic Sciences
Contents· 44 sections
- 1: DNA
- 1.1: DNA as Genetic Material
- 1.2: Structure of DNA and RNA
- 1.3: Bacterial Restriction Modification system
- 1.4: DNA Modifying Enzymes
- 1.5: DNA Replication - Introduction to Prokaryotic replication
- 1.6: DNA Supercoiling and Topoisomerases
- 2: Bacteria
- 2.1: The Bacteria - An Introduction
- 2.2: Bacterial Growth and Reproduction
- 2.3: Extrachromosomal elements plasmids selectable markers
- 2.4: Central Dogma and the Genetic Code
- 2.5: Gene and Operon
- 2.6: The lac Operon CAP site DNA footprinting
- 2.7: Transcriptional Regulation: Transcription termination the trp operon
- 2.8: mRNA Translation
- 3. Biotechnology 1
- 3.1: Gel Electrophoresis
- 3.2: DNA Sequence Analysis
- 3.3: Polymerase Chain Reaction (PCR) and Cloning of PCR Products
- 3.4: Prokaryotic Expression Vectors
- 3.5: Protein Sequencing Peptide Mapping Synthetic Genes
- 3.6: cDNA and Genomic Libraries
- 4. Biotechnology 2
- 4.1: Protein Purification
- 4.2: M13 Phage
- 4.3: M13 Phage Display Libraries
- 4.4: SELEX (Selective Evolution of Ligands by Exponential Enrichment)
- 4.5: Protein-Protein Recognition Probed Using a Yeast Transcriptional Activator System
- 4.6: Molecular Imprinting
- 5. Lab Notes Part 1
- 5.1: Basic practices and techniques Laboratory notebooks Presentation of data Sections of a scientific report
- 5.2: Spectrophotometry
- 5.3: Quantification of Protein Concentration
- 5.4: Chromatography
- 5.5: Gel Electrophoresis of Proteins
- 5.6: Introduction to Protein Purification
- 6. Lab Notes Part 2
- 6.1: Genetic Transformation (using bacteria and the pGLO plasmid)
- 6.2: Enzyme kinetics
- 6.3: Ligand binding
- 6.4: Restriction Mapping
- 6.5: Polymerase Chain Reaction (PCR)
- 6.6: Use of PC and internet for biochemical research