External
Lab manual : Protein Biomanufacturing and Biochemistry (Biot274 IVC)
- Author
- Victor Pham
- Publisher
- Irvine Valley College
- Source
- LibreTexts
- Language
- EN
- Category
- Basic Sciences
Contents· 12 sections
- 01: Activity 1-1 - Protein Extraction Using Bacterial Cell Lysis
- 02: Activity 1-2 - Bradford Assay Measuring Protein Concentration
- 03: Activity 1-3 - Beers Law Spectrophotometric Analysis
- 04: Activity 1-4 - Analysis of Enzymatic Activities of Cell-Free Extracts
- 05: Activity 2-1 - Purification via Ammonium Sulfate Saturation of Cell-Free Extract
- 06: Activity 2-2 - Dialysis for Enzyme Purification
- 07: Activity 2-3 - Size Exclusion Gel Filtration Purification of Enzymes
- 08: Activity 2-4 - Verification of Protein Purification via Polyacrylamide Gel Electrophoresis
- 09: Activity 3-1 - Project Instructions - Investigating Enzyme Kinetics
- 10: Activity 3-1 - Michaelis-Menten Analysis of Substrate Concentration on Reaction Rate
- 11: Activity 3-2 - Determining the IC of Inhibitor
- 12: Activity 3-3 - Identifying the Type of Enzyme Inhibition