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OER - BIOL 2420 Laboratory Manual

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OER - BIOL 2420 Laboratory Manual

Biology
Author
Sherry Smith
Publisher
North Central Texas College
Source
LibreTexts
License
CC BY
Language
EN
Category
Basic Sciences
Read online Download PDF (open licence)
Contents· 200 sections
  1. 01: Section One - Getting Started In The Microbiology Laboratory
  2. 1.01: Exercise 1 - Laboratory Safety
  3. 1.1.01: Microbiology Safety Guidelines
  4. 1.1.02: Laboratory Safety Equipment
  5. 1.1.03: Exercise 1 Lab Wrap-Up
  6. 1.02: Exercise 2 - Microscopy
  7. 1.2.01: Transporting the Microscope
  8. 1.2.02: Identifying the Components of the Compound Microscope
  9. 1.2.03: Calculating Total Magnification
  10. 1.2.04: Using the Compound Microscope
  11. 1.2.4.01: Observing the Letter e
  12. 1.2.4.02: Observing a whitefish blastodisc
  13. 1.2.4.03: Observing a human blood smear
  14. 1.2.4.04: Observing Balantidium coli
  15. 1.2.4.05: Observing a bacterial smear
  16. 1.2.05: Exercise 2 Lab Wrap-Up
  17. 1.03: Exercise 3 - Growth Media and Inoculating Tools
  18. 1.3.01: Types of Media - Chemical Composition
  19. 1.3.02: Types of Media - Physical Characteristics
  20. 1.3.03: Types of Media Functional Application Classifications
  21. 1.3.04: Inoculating Tools
  22. 1.3.05: Exercise 3 Lab Wrap-Up
  23. 1.04: Exercise 4 - Aseptic Techniques and Aseptic Transfer of Bacteria
  24. 1.4.01: Aseptic Technique and Subculturing Bacteria
  25. 1.4.02: Becoming Familiar with Aseptic Techniques
  26. 1.4.03: Aseptically Transferring Bacteria From Broth Culture to Sterile Broth
  27. 1.4.04: Aseptically Transferring Bacteria From Broth Culture to Sterile Agar Slant
  28. 1.4.05: Aseptically Transferring Bacteria From Broth Culture to Sterile Agar Plate
  29. 1.4.06: Exercise 4 Lab Wrap-Up
  30. 1.05: Exercise 5 - Isolation Techniques
  31. 1.5.01: Streak Plate
  32. 1.5.02: Performing the Streak Plate
  33. 1.5.03: Exercise 5 Lab Wrap-Up
  34. 1.06: Section One Review
  35. 1.6.01: Matching
  36. 1.6.02: Fill-In-The-Blank
  37. 1.6.03: True False
  38. 1.6.04: Multiple Choice Multiple Select
  39. 02: Section Two - Visualizing Bacteria (Staining Bacteria)
  40. 2.01: Exercise 6 - Bacterial Cellular Morphology and Structure
  41. 2.1.01: Cellular Morphology and Structure
  42. 2.1.02: Bacterial Cellular Arrangement
  43. 2.1.03: Exercise 6 Lab Wrap-Up
  44. 2.02: Exercise 7 - Specimen Preparation and Simple Staining
  45. 2.2.01: Specimen Preparation and Simple Staining
  46. 2.2.02: Cheek Smear Activity
  47. 2.2.03: Bacterial Smear Activity
  48. 2.2.04: Exercise 7 Lab Wrap-Up
  49. 2.03: Exercise 8 - Differential Staining- Gram Staining
  50. 2.3.01: Gram Stain
  51. 2.3.02: Gram Stain Activity
  52. 2.3.03: Smear Visualization
  53. 2.3.04: Exercise 8 Lab Wrap-Up
  54. 2.04: Exercise 9 - Differential Staining- Acid-Fast Stain
  55. 2.4.01: Acid-Fast Stain
  56. 2.4.02: Acid-Fast Stain Activity
  57. 2.4.03: Smear Visualization
  58. 2.4.04: Exercise 9 Lab Wrap-Up
  59. 2.05: Exercise 10 - Structural Stain- Endospore Stain
  60. 2.5.01: Endospore Stain
  61. 2.5.02: Endospore Stain Activity
  62. 2.5.03: Smear Visualization
  63. 2.5.04: Exercise 10 Lab Wrap-Up
  64. 2.06: Exercise 11 - Structural Stain- Capsule Stain
  65. 2.6.01: Capsule Stain
  66. 2.6.02: Capsule Stain Activity
  67. 2.6.03: Smear Visualization
  68. 2.6.04: Exercise 11 - Lab Wrap-Up
  69. 2.07: Section Two Review
  70. 2.7.01: Matching
  71. 2.7.02: Fill-In-The-Bank
  72. 2.7.03: True False
  73. 2.7.04: Multiple Choice Multiple Select
  74. 03: Section Three Assessing Metabolic Characteristics of Bacteria (Biochemical Tests Selective and Differential Media)
  75. 3.01: Exercise 12 - Carbohydrate Fermentation Tests
  76. 3.1.01: Carbohydrate Fermentation Test
  77. 3.1.02: Carbohydrate Fermentation Test Activity
  78. 3.1.03: Exercise 12 - Lab Wrap-Up
  79. 3.02: Exercise 13 - Triple Sugar Iron Agar Test
  80. 3.2.01: Triple Sugar Iron Agar Test
  81. 3.2.02: Triple Sugar Iron Agar Test Activity
  82. 3.2.03: Exercise 13 - Lab Wrap-Up
  83. 3.03: Exercise 14 - SIM Medium Test
  84. 3.3.01: SIM Medium Test
  85. 3.3.02: SIM Medium Test Activity
  86. 3.3.03: Exercise 14 - Lab Wrap-Up
  87. 3.04: Exercise 15 - IMViC Tests
  88. 3.4.01: Indole Test
  89. 3.4.02: Indole Test Activity
  90. 3.4.03: MRVP
  91. 3.4.04: Methyl Red (MR) and Voges-Proskauer (VP) Test Activity
  92. 3.4.05: Citrate Test
  93. 3.4.06: Citrate Test Activity
  94. 3.4.07: Exercise 15 - Lab Wrap-Up
  95. 3.05: Exercise 16 - Respiration Tests
  96. 3.5.01: Oxidase Test
  97. 3.5.02: Oxidase Test Activity
  98. 3.5.03: Catalase Test
  99. 3.5.04: Catalase Test Activity
  100. 3.5.05: Nitrate Reduction Test
  101. 3.5.06: Nitrate Reduction Test Activity
  102. 3.5.07: Exercise 16 - Lab Wrap-Up
  103. 3.06: Exercise 17 - Urease Test
  104. 3.6.01: Urease Test
  105. 3.6.02: Urease Test Activity
  106. 3.6.03: Exercise 17 - Lab Wrap-Up
  107. 3.07: Exercise 18 - Coagulase Test
  108. 3.7.01: Coagulase Test
  109. 3.7.02: Coagulase Test Activity
  110. 3.7.03: Exercise 18 - Lab Wrap-Up
  111. 3.08: Exercise 19 - Casease Test
  112. 3.8.01: Casease Test
  113. 3.8.02: Casease Test Activity
  114. 3.8.03: Exercise 19 - Lab Wrap-Up
  115. 3.09: Exercise 20 - Starch Hydrolysis Test
  116. 3.9.01: Starch Hydrolysis Test
  117. 3.9.02: Starch Hydrolysis Test Activity
  118. 3.9.03: Exercise 20 - Lab Wrap-Up
  119. 3.10: Exercise 21 - Blood Agar
  120. 3.10.01: Blood Agar Test
  121. 3.10.02: Blood Agar Test Activity
  122. 3.10.03: Exercise 21 - Lab Wrap-Up
  123. 3.11: Exercise 22 MacConkey Agar
  124. 3.11.01: MacConkey Agar
  125. 3.11.02: MacConkey Agar Activity
  126. 3.11.03: Exercise 22 - Lab Wrap-Up
  127. 3.12: Exercise 23 - Mannitol Salt Agar
  128. 3.12.01: Mannitol Salt Agar Test
  129. 3.12.02: Mannitol Salt Agar Test Activity
  130. 3.12.03: Exercise 23 - Lab Wrap-Up
  131. 3.13: Section Three Review
  132. 3.13.01: Matching
  133. 3.13.02: Fill-In-The-Blank
  134. 3.13.03: True False
  135. 3.13.04: Multiple Choice Multiple Select
  136. 04: Section Four - Enumerating Bacteria
  137. 4.01: Exercise 24 - Viable Plate Counts
  138. 4.1.01: Serial Dilutions and Viable Plate Counts
  139. 4.1.02: Serial Dilutions and Viable Plate Counts Activity
  140. 4.1.03: Exercise 24 - Lab Wrap-Up
  141. 4.02: Exercise 25- Direct Microscopic Count
  142. 4.2.01: Direct Microscopic Count
  143. 4.2.02: Direct Microscopic Count Activity
  144. 4.2.03: Exercise 25 - Lab Wrap-Up
  145. 4.03: Section Four Review
  146. 4.3.01: Matching
  147. 4.3.02: Fill-In-The-Blank
  148. 4.3.03: True False
  149. 4.3.04: Multiple Choice Multiple Select
  150. 05: Section Five - Factors Affecting Microbial Growth
  151. 5.01: Exercise 26 - Effect of Temperature on Microbial Growth
  152. 5.1.01: Temperature and Microbial Growth
  153. 5.1.02: Assessing Effects of Temperature on Microbial Growth Activity
  154. 5.1.03: Assessing Effects of Temperature on Phenotypic Expression Activity
  155. 5.1.04: Exercise 26 - Lab Wrap-Up
  156. 5.02: Exercise 27 - Effect of pH on Microbial Growth
  157. 5.2.01: pH and Microbial Growth
  158. 5.2.02: Assessing Effects of pH on Microbial Growth Activity
  159. 5.2.03: Exercise 27 - Lab Wrap-Up
  160. 5.03: Exercise 28 - Effect of Antiseptics and Disinfectants on Microbial Growth
  161. 5.3.01: Antiseptics and Disinfectants
  162. 5.3.02: Assessing the Effects of Antiseptics and Disinfectants on Microbial Growth Activity
  163. 5.3.03: Exercise 28 - Lab Wrap-Up
  164. 5.04: Exercise 29 - Antimicrobial Sensitivity Test (Kirby-Bauer Method)
  165. 5.4.01: Antimicrobial Susceptibility Test
  166. 5.4.02: Antimicrobial Sensitivity Test (Kirby-Bauer Method) Activity
  167. 5.4.03: Exercise 29 - Lab Wrap-Up
  168. 5.05: Section Five Review
  169. 5.5.01: Matching
  170. 5.5.02: Fill-In-The-Blank
  171. 5.5.03: True False
  172. 5.5.04: Multiple Choice Multiple Select
  173. 06: Section Six - Eukaryotic Pathogens (A Survey of Fungal Protozoan and Helminth Pathogens)
  174. 6.01: Exercise 30 - Fungal Pathogens
  175. 6.1.01: Fungi
  176. 6.1.02: Survey of Clinically Significant Yeasts
  177. 6.1.03: Survey of Clinically Significant Molds
  178. 6.1.04: Exercise 30 - Lab Wrap-Up
  179. 6.02: Exercise 31 - Protozoan Parasites
  180. 6.2.01: Protozoans
  181. 6.2.02: Subphylum Sarcodina (Amoeboids)
  182. 6.2.03: Subphylum Mastigophora (Flagellates)
  183. 6.2.04: Phylum Ciliata (Cilates)
  184. 6.2.05: Phylum Apicomplexa (Sporozoa)
  185. 6.2.06: Exercise 31 - Lab Wrap-Up
  186. 6.03: Exercise 32 - Helminth Pathogens
  187. 6.3.01: Helminths
  188. 6.3.02: Trematodes (Flukes)
  189. 6.3.03: Survey of Trematodes
  190. 6.3.04: Cestodes (Tapeworms)
  191. 6.3.05: Survey of Cestodes
  192. 6.3.06: Nematodes (Roundworms)
  193. 6.3.07: Survey of Nematodes
  194. 6.3.08: Exercise 32 - Lab Wrap-Up
  195. 6.04: Section Six Review
  196. 6.4.01: Matching
  197. 6.4.02: Fill-In-The-Blank
  198. 6.4.03: True-False
  199. 6.4.04: Multiple Choice Multiple Select
  200. 6.4.05: Identifying Parasites