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Quantitative NMR

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Quantitative NMR

Quantitative nuclear magnetic resonance (Q-NMR) measurements are especially useful for analysis of complex samples where high specificity is needed or when a pure standard of the analyte is not available. This text provides a brief overview of basic NMR theory, a tutorial discussing the practical aspects of Q-NMR, a virtual experiment utilizing an NMR simulator, an exercise that makes use of real NMR data you can download and analyze, and a Q-NMR lab experiments.

Physical and Theoretical Chemistry Textbook MapsChemistry
Author
Cynthia K. Larive & Albert K. Korir
Publisher
University of California, Riverside & Drury University
Source
LibreTexts
License
CC BY-NC-SA
Language
EN
Category
Basic Sciences
Read online Download PDF (open licence)
Contents· 39 sections
  1. 01: Basic NMR Theory
  2. 1.01: What is spin
  3. 1.02: How does absorption of energy generate an NMR spectrum
  4. 1.03: How does the population difference in NMR compare to the difference between electronic ground and excited states
  5. 1.04: What is chemical shift and how does it relate to resonance frequency
  6. 1.05: What is precession
  7. 1.06: How does precession generate the macroscopic magnetization (Mo)
  8. 1.07: How can the nuclear spins be manipulated to generate the NMR spectrum
  9. 1.08: What is the tip angle
  10. 1.09: What is the Free Induction Decay
  11. 1.10: How do T and T relaxation affect NMR spectra
  12. 1.11: Where should I look to learn more about NMR
  13. 02: Practical Aspects of Q-NMR
  14. 2.01: How do I choose a reference standard for my Q-NMR analysis
  15. 2.02: How is the internal standard used to quantify the concentration of my analyte
  16. 2.03: What sample considerations are important
  17. 2.04: How do I choose the right acquisition parameters for a quantitative NMR measurement
  18. 2.05: Effects of Tip Angle in Quantitative NMR Experiments
  19. 2.06: What data processing considerations are important for obtaining accurate and precise results
  20. 2.07: References
  21. 03: Virtual Experiment
  22. 3.01: Virtual Laboratory
  23. 04: Q-NMR Experiment
  24. 4.01: Prelab Exercises
  25. 4.02: Background
  26. 4.03: Dry Lab
  27. 4.04: Wet Lab
  28. 05: Q-NMR Applications
  29. 5.01: Q-NMR for purity determination of macrolide antibiotic reference standards- Comparison with the mass balance method
  30. 5.02: Determining Enantiomeric or Isomeric Purity of Active Pharmaceutical Ingredients
  31. 5.03: Q-NMR for Analysis and Characterization in Vaccine Preparations
  32. 5.04: Q-NMR-Based Metabonomics of Blood Samples
  33. 5.05: Q-NMR for Time Course Evolution of Malic and Lactic Acid
  34. 06: Instructor's Guide
  35. 6.01: Basic Theory Concept Questions
  36. 6.02: Answers to Questions in the Basic Theory section
  37. 6.03: Practical Aspects Concept Questions
  38. 6.04: Answers to Questions in the Practical Aspects section
  39. 6.05: Q-NMR Drylab