External
Lab manual: Molecular biology and Genetic Engineering (Biot 275)
- Author
- Victor Pham
- Publisher
- Irvine Valley College
- Source
- LibreTexts
- License
- CC BY-NC
- Language
- EN
- Category
- Basic Sciences
Contents· 15 sections
- 01: Activity 1-1 Data on Human Traits
- 02: Activity 1-2 - Human Genetic Inheritance and Diseases
- 03: Activity 1-3 - Genetic evolution and Identifying Homologs
- 04: Activity 1-4 - PowerPoint Presentation on the Bioinformatic Evolution and function of proteins
- 05: Activity 2-0 - Introduction to Plasmid and Enzyme p450
- 06: Activity 2-1 - Plasmids Isolation
- 07: Activity 2-2 - DNA Analysis via Restriction Enzymes
- 08: Project 2-3 - Identifying Plasmid Restriction Mapping Using Agarose Gel Electrophoresis
- 09: Activity 2-4 - Bacterial Transformation with Engineered Plasmid
- 10: Activity 3-1 - Identifying Genetically Modified Organisms Through DNA Analysis
- 11: Activity 3-2 - Primer Design and Sequence Verification Using Bioinformatics
- 12: Activity 4-1 - RNA Extraction via Guanidine Thiocyanate and Phenol
- 13: Activity 4-2 - Reverse Transcription Polymerase Chain Reaction
- 14: Activity 4-3 -Real-Time Quantitative Polymerase Chain Reaction
- 15: Activity 4-4 - DNA Sequencing with SeqStudio Genetic Analyzer