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Introduction to Biotechnology Laboratory Manual

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Introduction to Biotechnology Laboratory Manual

Regardless if you work at a biotechnology company or in an academic research lab, keeping a lab notebook is a requirement. The Food and Drug Administration’s (FDA) handbook states, "if it isn't written down, it wasn't done." The lab notebook is considered a legal document and can be used in court to settle patent disputes or to report a specialist’s finding in paternity suits or criminal cases. Often times, the lab notebook is used as a starting point for other scientists who work in the lab.

BiotechnologyBiology
Author
Donna Barron
Publisher
Hudson Valley Community College
Source
LibreTexts
License
CC BY-NC-SA
Language
EN
Category
Basic Sciences
Read online Download PDF (open licence)
Contents· 62 sections
  1. 01: Lab Safety and Lab Notebook
  2. 1.01: Student Learning Outcomes
  3. 1.02: Introduction
  4. 1.03: PART I- Setting Up a Legal Scientific Notebook
  5. 1.04: PART II- Lab Safety
  6. 1.05: Study Questions
  7. 1.06: PUZZLE- Lab Safety and Lab Notebooks
  8. 02: Metrics and Measurements
  9. 2.01: Student Learning Outcomes
  10. 2.02: PART I- Metrics
  11. 2.03: Part II- Measuring Using The Metric System
  12. 2.04: Study Questions
  13. 03: Micropipetting
  14. 3.01: Student Learning Outcomes
  15. 3.02: Introduction To Micropipetting
  16. 3.03: Part I- Choosing And Setting The Micropipette
  17. 3.04: Part II- Pipetting Practice
  18. 3.05: Study Questions
  19. 04: pH and Buffers
  20. 4.01: Student Learning Outcomes
  21. 4.02: Introduction
  22. 4.03: Prelab Reading Assignment- Chemistry Review
  23. 4.04: Activity 1- Measuring pH
  24. 4.05: Activity 2- Preparation of an Acetate Buffer
  25. 4.06: Activity 3- Effect of Adding Acid and Base to Acetate Buffer
  26. 4.07: Study Questions
  27. 05: Paternity and Gel Electrophoresis
  28. 5.01: Student Learning Outcomes
  29. 5.02: Part I- Agarose Gel Electrophoresis
  30. 5.03: Part II- Paternity Case- Who is the Father of My Kittens
  31. 5.04: Study Questions
  32. 06: Restriction Digest and Gel Electrophoresis (New)
  33. 6.01: Student Learning Outcomes
  34. 6.02: Introduction
  35. 6.03: Part I- Restriction Digest
  36. 6.04: Part II- Casting Agarose Gel
  37. 6.05: Part III- Practice Pipetting
  38. 6.06: Part IV- Gel Electrophoresis
  39. 6.07: Study Questions
  40. 07: Transformation
  41. 7.01: Student Learning Outcomes
  42. 7.02: Introduction
  43. 7.03: Transforming Bacteria with Plasmids
  44. 7.04: Study Questions
  45. 08: Column Chromatography (Rev)
  46. 8.01: Student Learning Outcomes
  47. 8.02: Introduction
  48. 8.03: Pre Lab Activity- Think Pair Share-Discuss What You Know
  49. 8.04: Part I- Lysis of Bacterial Cells
  50. 8.05: Part II- Separating the Green Fluorescent Protein with Column Chromatography
  51. 8.06: Study Questions
  52. 09: Serial Dilutions and Standard Curve with a Microplate
  53. 9.01: Student Learning Outcomes
  54. 9.02: Part I- Serial Dilutions
  55. 9.03: Part II- Standard Curves
  56. 9.04: Study Questions
  57. 10: Good Manufacturing Practices (GMP) with New Table
  58. 10.01: Student Learning Outcomes
  59. 10.02: Part I- Review Terms and Background Information
  60. 10.03: Part II- Train and Prepare for a Production Run
  61. 10.04: Part III- Production and Documentation
  62. 10.05: Study Questions