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Microbiology Laboratory Manual

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Microbiology Laboratory Manual

This text is designed for instructional laboratories of undergraduate general microbiology courses. Basic microbiological laboratory approaches are emphasized, as well as the diversity of microbes. Morphologies, physiologies, methods of microbial isolation, identification, and characterization are explored. The manual includes an unknown identification project with instructions for writing a scientific report detailing the project and its results.

MicrobiologyBiology
Author
Rosanna Hartline
Publisher
West Hills College Lemoore
Source
LibreTexts
License
CC BY-NC-SA
Language
EN
Category
Basic Sciences
Read online Download PDF (open licence)
Contents· 147 sections
  1. 01: Labs
  2. 1.01: Laboratory Safety
  3. 1.02: Media Preparation
  4. 1.03: The Myth of Spontaneous Generation
  5. 1.04: Microscopy
  6. 1.05: Get to Know the Microscope and Microbes
  7. 1.06: Molecules of Life
  8. 1.07: Aseptic Technique
  9. 1.08: Plating on Petri Plates for Isolation
  10. 1.09: Simple Stain
  11. 1.10: Gram Stain
  12. 1.11: Prokaryotic Cells
  13. 1.12: Endospore Stain
  14. 1.13: Capsule Stain
  15. 1.14: Acid-Fast Stain
  16. 1.15: Determination of Bacterial Numbers
  17. 1.16: Eukaryotic Cells
  18. 1.17: Starch Hydrolysis
  19. 1.18: Catalase Test
  20. 1.19: Cytochrome c Oxidase
  21. 1.20: Citrate Test
  22. 1.21: Bacterial Oxygen Requirements
  23. 1.22: Fermentation
  24. 1.23: SIM Deep Tests
  25. 1.24: Coagulase Test
  26. 1.25: Gelatin Hydrolysis
  27. 1.26: Nitrate Reduction
  28. 1.27: MR-VP Tests
  29. 1.28: EMB Agar
  30. 1.29: Mannitol Salt Agar
  31. 1.30: DNA RNA and DNA Replication
  32. 1.31: PCR
  33. 1.32: DNA Fingerprinting
  34. 1.33: Bacterial Transformation
  35. 1.34: Protozoan Parasites
  36. 1.35: Helminth Parasites
  37. 1.36: Fungal Parasites
  38. 1.37: Viruses and Viral Epidemic Simulation
  39. 1.38: Virus Bioassay
  40. 1.39: Control of Microbial Growth
  41. 1.40: Bacterial Susceptibility to Antibiotics (Kirby-Bauer Test)
  42. 1.41: Human Microbiome
  43. 1.42: Unknown Bacteria Identification Project
  44. 1.43: Unknown Bacteria Identification Project Report
  45. 02: Exercise Answers
  46. 2.01: Exercise 2.1
  47. 2.02: Exercise 2.2
  48. 2.03: Exercise 2.3
  49. 2.04: Exercise 2.4
  50. 2.05: Exercise 2.5
  51. 2.06: Exercise 4.1
  52. 2.07: Exercise 4.2
  53. 2.08: Exercise 4.3
  54. 2.09: Exercise 4.4
  55. 2.10: Exercise 4.5
  56. 2.11: Exercise 4.6
  57. 2.12: Exercise 5.1
  58. 2.13: Exercise 5.2
  59. 2.14: Exercise 5.3
  60. 2.15: Exercise 5.4
  61. 2.16: Exercise 5.5-A
  62. 2.17: Exercise 5.5-B
  63. 2.18: Exercise 5.5-C
  64. 2.19: Exercise 5.5-D
  65. 2.20: Exercise 5.5-E
  66. 2.21: Exercise 5.5-F
  67. 2.22: Exercise 5.5-G
  68. 2.23: Exercise 5.5-H
  69. 2.24: Exercise 5.5-I
  70. 2.25: Exercise 5.5-J
  71. 2.26: Exercise 5.5-K
  72. 2.27: Exercise 5.5-L
  73. 2.28: Exercise 5.6-ocular lens
  74. 2.29: Exercise 5.6-revolving nosepiece
  75. 2.30: Exercise 5.6-arm
  76. 2.31: Exercise 5.6-stage control
  77. 2.32: Exercise 5.6-base
  78. 2.33: Exercise 5.6-course focus
  79. 2.34: Exercise 5.6-fine focus
  80. 2.35: Exercise 5.6-light source illuminator
  81. 2.36: Exercise 5.6-objective lens
  82. 2.37: Exercise 5.6-stage
  83. 2.38: Exercise 5.6-stage clip
  84. 2.39: Exercise 5.6-diaphragm
  85. 2.40: Exercise 5.6-eyepiece
  86. 2.41: Exercise 6.1-1
  87. 2.42: Exercise 6.1-2
  88. 2.43: Exercise 6.1-3
  89. 2.44: Exercise 6.1-4
  90. 2.45: Exercise 6.1-5
  91. 2.46: Exercise 6.1-6
  92. 2.47: Exercise 6.1-7
  93. 2.48: Exercise 6.1-8
  94. 2.49: Exercise 10.1
  95. 2.50: Exercise 10.2
  96. 2.51: Exercise 10.3
  97. 2.52: Exercise 10.4
  98. 2.53: Exercise 10.5
  99. 2.54: Exercise 10.6
  100. 2.55: Exercise 15.1
  101. 2.56: Exercise 15.2
  102. 2.57: Exercise 15.3
  103. 2.58: Exercise 15.4
  104. 2.59: Exercise 15.5
  105. 2.60: Exercise 15.6
  106. 2.61: Exercise 22.1
  107. 2.62: Exercise 22.2
  108. 2.63: Sample Project Report
  109. 03: Instructor Setup
  110. 3.01: Myth of Spontaneous Generation
  111. 3.02: Get to Know the Microscope and Microbes
  112. 3.03: Molecules of Life
  113. 3.04: Aseptic Technique
  114. 3.05: Plating on Petri Plates for Isolation
  115. 3.06: Simple Stain
  116. 3.07: Gram Stain
  117. 3.08: Endospore Stain
  118. 3.09: Capsule Stain
  119. 3.10: Acid-Fast Stain
  120. 3.11: Determination of Bacterial Numbers
  121. 3.12: Eukaryotic Cells
  122. 3.13: Starch Hydrolysis
  123. 3.14: Catalase Test
  124. 3.15: Cytochrome C Oxidase
  125. 3.16: Citrate Test
  126. 3.17: Bacterial Oxygen Requirements
  127. 3.18: Fermentation
  128. 3.19: SIM Deep Tests
  129. 3.20: Coagulase Test
  130. 3.21: Gelatin Hydrolysis
  131. 3.22: Nitrate Reduction
  132. 3.23: MR-VP Tests
  133. 3.24: EMB Agar
  134. 3.25: Mannitol Salt Agar
  135. 3.26: DNA RNA and DNA Replication
  136. 3.27: PCR
  137. 3.28: DNA Fingerprinting
  138. 3.29: Bacterial Transformation
  139. 3.30: Protozoan Parasites
  140. 3.31: Helminth Parasites
  141. 3.32: Fungal Parasites
  142. 3.33: Viral Epidemic
  143. 3.34: Virus Bioassay
  144. 3.35: Control of Microbial Growth
  145. 3.36: Bacterial Susceptibility to Antibiotics (Kirby-Bauer Test)
  146. 3.37: Human Microbiome
  147. 3.38: Unknown Bacteria Identification Project