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Reyna Genetics Lab - Synthetic Biology CURE
The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions.
- Author
- Nathan Reyna
- Publisher
- Ouachita Baptist University
- Source
- LibreTexts
- Language
- EN
- Category
- Basic Sciences
Contents· 13 sections
- 01: Inoculation
- 02: Lab Safety and Laboratory Notebook
- 03: Tips for Organizing the Lab for Large Classes
- 04: Visual Representation of the Protocol
- 05: Plasmid DNA Extraction (Mini-Prep)
- 06: Appendix III- Using the Nanodrop
- 07: DNA Double Digestion
- 08: Gel Loading and Running
- 09: Ligation of Products
- 10: Cell Transformation
- 11: Appendix I- Antibiotics and Color Coding
- 12: Appendix V- Common Agarose Gel Questions and Tips
- 13: Before you begin a semester overview