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MB352 General Microbiology Laboratory 2021

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MB352 General Microbiology Laboratory 2021

Biology
Author
Alice Lee
Publisher
North Carolina State University
Source
LibreTexts
Language
EN
Category
Basic Sciences
Read online Download PDF (open licence)
Contents· 55 sections
  1. 01: Laboratory Safety
  2. 1.01: Safety Procedures for the Microbiology Laboratory
  3. 1.02: Biosafety levels and PPE
  4. 1.03: Review Questions
  5. Review Questions II
  6. Review questions III
  7. 02: Cultivation of Microbes
  8. 2.01: Introduction Growth Media
  9. 2.02: Introduction to Bacterial Growth and Aseptic Techniques
  10. 2.03: Examples of Bacterial Growth Characteristics in Broths Slants and Plates
  11. 2.04: Lab Procedures- Prepare solid media Aseptic Technique T-streaking
  12. 2.05: Results
  13. 2.06: Review Questions
  14. 03: Microscopy
  15. 3.01: Introduction to the Microscope
  16. 3.02: Comparison of Sizes and Shapes of Microorganisms
  17. 3.03: Lab Procedures- Operating a Microscope
  18. 3.04: Results
  19. 3.05: Review Questions
  20. 04: Staining Techniques
  21. 4.01: Introduction to Staining
  22. 4.02: Specialized Bacterial Staining Techniques
  23. 4.03: Lab Procedures- Bacterial Smear Simple and Gram Staining
  24. 4.04: Results
  25. 4.05: Review Questions
  26. 05: Enumeration of Bacteria
  27. 5.01: Introduction to Enumeration of Bacteria
  28. 5.02: Lab Procedures- How to operate a Pipettor
  29. 5.03: Lab Procedures- Viable Plate count
  30. 5.04: Results
  31. 5.05: Review Questions
  32. 06: Microbial Physiology
  33. 6.01: Introduction to Oxygen Requirements
  34. 6.1.01: Determining Oxygen Requirements and Anaerobes
  35. 6.02: Temperature pH and Osmotic Requirements
  36. 6.03: Bacterial Growth Dynamics
  37. 6.04: Bacteriophages
  38. 6.05: Lab Procedures- Testing Oxygen requirements
  39. 6.06: Lab Procedures- Plaque Assay
  40. 6.07: Results
  41. 6.08: Review Questions
  42. 07: Microbial Metabolism
  43. 7.01: Introduction to Biochemical Tests Part I
  44. 7.02: Introduction to Biochemical Tests Part II
  45. 7.03: Lab Procedures- Biochemical Tests
  46. 7.04: Results
  47. 7.05: Review Questions
  48. 08: Bacterial Identification
  49. 8.01: Introduction to Bacterial Identification using Culture Media
  50. 8.02: Introduction to Bacterial Identification using Enterotube test
  51. 8.03: Introduction to Bacterial Identification using Genotypic methods
  52. 8.04: Lab Procedures- Enterotube inoculation
  53. 8.05: Lab Procedures- PCR and Gel Electrophoresis
  54. 8.06: Results
  55. 8.07: Review Questions