Nguồn ngoài
Lab Manual: Synthetic Biology Protocols
The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions.
- Tác giả
- Nathan Reyna, Ruth Plymale, & Kristine Johnson
- Nhà xuất bản
- Ouachita Babtist University & University of New Hampshire
- Nguồn
- LibreTexts
- Ngôn ngữ
- EN
- Danh mục
- Khoa học cơ bản
Mục lục· 29 mục
- 2.3.01: Getting Started-Instructor's Guide
- 2.3.1.01: Making Media (LB Broth and Agar)
- 2.3.1.02: Adding Antibiotics
- 2.3.1.03: Making Linearized Backbone PCR Protocol
- 2.3.1.04: DNA Extraction Supplies
- 2.3.1.05: Double Digestion Supplies
- 2.3.1.06: Gel Loading Running Supplies
- 2.3.1.07: DNA Ligation Supplies
- 2.3.1.08: Transformation Supplies
- 2.3.1.09: Inoculation Supplies
- 2.3.1.10: Glycerol Stock Supplies
- 2.3.1.11: Linearizing Backbone Supplies
- 2.3.1.12: Tips for Organizing the Lab for Large Classes
- 2.3.1.13: Before you begin a semester overview
- 2.3.02: Student Lab Manual- Protocols
- 2.3.2.01: Visual Representation of the Protocol
- 2.3.2.02: Plasmid DNA Extraction (Mini-Prep)
- 2.3.2.03: DNA Double Digestion
- 2.3.2.04: Gel Loading and Running
- 2.3.2.05: Ligation of Products
- 2.3.2.06: Cell Transformation
- 2.3.2.07: Inoculation
- 2.3.03: Appendices- Equipment Use
- 2.3.3.01: Appendix I- Antibiotics and Color Coding
- 2.3.3.02: Appendix II- Using the Denovix
- 2.3.3.03: Appendix III- Using the Nanodrop
- 2.3.3.04: Appendix IV- Using the Qubit
- 2.3.3.05: Appendix V- Common Agarose Gel Questions and Tips
- 2.3.3.06: Appendix IV- Preparing Glycerol Stocks