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OER - BIOL 2420 Laboratory Manual

Biology

Tác giả: Sherry Smith
Nhà xuất bản: North Central Texas College
Nguồn: LibreTexts
Giấy phép: CC BY
Ngôn ngữ: EN
Danh mục: Khoa học cơ bản

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Mục lục· 200 mục

01: Section One - Getting Started In The Microbiology Laboratory
1.01: Exercise 1 - Laboratory Safety
1.1.01: Microbiology Safety Guidelines
1.1.02: Laboratory Safety Equipment
1.1.03: Exercise 1 Lab Wrap-Up
1.02: Exercise 2 - Microscopy
1.2.01: Transporting the Microscope
1.2.02: Identifying the Components of the Compound Microscope
1.2.03: Calculating Total Magnification
1.2.04: Using the Compound Microscope
1.2.4.01: Observing the Letter e
1.2.4.02: Observing a whitefish blastodisc
1.2.4.03: Observing a human blood smear
1.2.4.04: Observing Balantidium coli
1.2.4.05: Observing a bacterial smear
1.2.05: Exercise 2 Lab Wrap-Up
1.03: Exercise 3 - Growth Media and Inoculating Tools
1.3.01: Types of Media - Chemical Composition
1.3.02: Types of Media - Physical Characteristics
1.3.03: Types of Media Functional Application Classifications
1.3.04: Inoculating Tools
1.3.05: Exercise 3 Lab Wrap-Up
1.04: Exercise 4 - Aseptic Techniques and Aseptic Transfer of Bacteria
1.4.01: Aseptic Technique and Subculturing Bacteria
1.4.02: Becoming Familiar with Aseptic Techniques
1.4.03: Aseptically Transferring Bacteria From Broth Culture to Sterile Broth
1.4.04: Aseptically Transferring Bacteria From Broth Culture to Sterile Agar Slant
1.4.05: Aseptically Transferring Bacteria From Broth Culture to Sterile Agar Plate
1.4.06: Exercise 4 Lab Wrap-Up
1.05: Exercise 5 - Isolation Techniques
1.5.01: Streak Plate
1.5.02: Performing the Streak Plate
1.5.03: Exercise 5 Lab Wrap-Up
1.06: Section One Review
1.6.01: Matching
1.6.02: Fill-In-The-Blank
1.6.03: True False
1.6.04: Multiple Choice Multiple Select
02: Section Two - Visualizing Bacteria (Staining Bacteria)
2.01: Exercise 6 - Bacterial Cellular Morphology and Structure
2.1.01: Cellular Morphology and Structure
2.1.02: Bacterial Cellular Arrangement
2.1.03: Exercise 6 Lab Wrap-Up
2.02: Exercise 7 - Specimen Preparation and Simple Staining
2.2.01: Specimen Preparation and Simple Staining
2.2.02: Cheek Smear Activity
2.2.03: Bacterial Smear Activity
2.2.04: Exercise 7 Lab Wrap-Up
2.03: Exercise 8 - Differential Staining- Gram Staining
2.3.01: Gram Stain
2.3.02: Gram Stain Activity
2.3.03: Smear Visualization
2.3.04: Exercise 8 Lab Wrap-Up
2.04: Exercise 9 - Differential Staining- Acid-Fast Stain
2.4.01: Acid-Fast Stain
2.4.02: Acid-Fast Stain Activity
2.4.03: Smear Visualization
2.4.04: Exercise 9 Lab Wrap-Up
2.05: Exercise 10 - Structural Stain- Endospore Stain
2.5.01: Endospore Stain
2.5.02: Endospore Stain Activity
2.5.03: Smear Visualization
2.5.04: Exercise 10 Lab Wrap-Up
2.06: Exercise 11 - Structural Stain- Capsule Stain
2.6.01: Capsule Stain
2.6.02: Capsule Stain Activity
2.6.03: Smear Visualization
2.6.04: Exercise 11 - Lab Wrap-Up
2.07: Section Two Review
2.7.01: Matching
2.7.02: Fill-In-The-Bank
2.7.03: True False
2.7.04: Multiple Choice Multiple Select
03: Section Three Assessing Metabolic Characteristics of Bacteria (Biochemical Tests Selective and Differential Media)
3.01: Exercise 12 - Carbohydrate Fermentation Tests
3.1.01: Carbohydrate Fermentation Test
3.1.02: Carbohydrate Fermentation Test Activity
3.1.03: Exercise 12 - Lab Wrap-Up
3.02: Exercise 13 - Triple Sugar Iron Agar Test
3.2.01: Triple Sugar Iron Agar Test
3.2.02: Triple Sugar Iron Agar Test Activity
3.2.03: Exercise 13 - Lab Wrap-Up
3.03: Exercise 14 - SIM Medium Test
3.3.01: SIM Medium Test
3.3.02: SIM Medium Test Activity
3.3.03: Exercise 14 - Lab Wrap-Up
3.04: Exercise 15 - IMViC Tests
3.4.01: Indole Test
3.4.02: Indole Test Activity
3.4.03: MRVP
3.4.04: Methyl Red (MR) and Voges-Proskauer (VP) Test Activity
3.4.05: Citrate Test
3.4.06: Citrate Test Activity
3.4.07: Exercise 15 - Lab Wrap-Up
3.05: Exercise 16 - Respiration Tests
3.5.01: Oxidase Test
3.5.02: Oxidase Test Activity
3.5.03: Catalase Test
3.5.04: Catalase Test Activity
3.5.05: Nitrate Reduction Test
3.5.06: Nitrate Reduction Test Activity
3.5.07: Exercise 16 - Lab Wrap-Up
3.06: Exercise 17 - Urease Test
3.6.01: Urease Test
3.6.02: Urease Test Activity
3.6.03: Exercise 17 - Lab Wrap-Up
3.07: Exercise 18 - Coagulase Test
3.7.01: Coagulase Test
3.7.02: Coagulase Test Activity
3.7.03: Exercise 18 - Lab Wrap-Up
3.08: Exercise 19 - Casease Test
3.8.01: Casease Test
3.8.02: Casease Test Activity
3.8.03: Exercise 19 - Lab Wrap-Up
3.09: Exercise 20 - Starch Hydrolysis Test
3.9.01: Starch Hydrolysis Test
3.9.02: Starch Hydrolysis Test Activity
3.9.03: Exercise 20 - Lab Wrap-Up
3.10: Exercise 21 - Blood Agar
3.10.01: Blood Agar Test
3.10.02: Blood Agar Test Activity
3.10.03: Exercise 21 - Lab Wrap-Up
3.11: Exercise 22 MacConkey Agar
3.11.01: MacConkey Agar
3.11.02: MacConkey Agar Activity
3.11.03: Exercise 22 - Lab Wrap-Up
3.12: Exercise 23 - Mannitol Salt Agar
3.12.01: Mannitol Salt Agar Test
3.12.02: Mannitol Salt Agar Test Activity
3.12.03: Exercise 23 - Lab Wrap-Up
3.13: Section Three Review
3.13.01: Matching
3.13.02: Fill-In-The-Blank
3.13.03: True False
3.13.04: Multiple Choice Multiple Select
04: Section Four - Enumerating Bacteria
4.01: Exercise 24 - Viable Plate Counts
4.1.01: Serial Dilutions and Viable Plate Counts
4.1.02: Serial Dilutions and Viable Plate Counts Activity
4.1.03: Exercise 24 - Lab Wrap-Up
4.02: Exercise 25- Direct Microscopic Count
4.2.01: Direct Microscopic Count
4.2.02: Direct Microscopic Count Activity
4.2.03: Exercise 25 - Lab Wrap-Up
4.03: Section Four Review
4.3.01: Matching
4.3.02: Fill-In-The-Blank
4.3.03: True False
4.3.04: Multiple Choice Multiple Select
05: Section Five - Factors Affecting Microbial Growth
5.01: Exercise 26 - Effect of Temperature on Microbial Growth
5.1.01: Temperature and Microbial Growth
5.1.02: Assessing Effects of Temperature on Microbial Growth Activity
5.1.03: Assessing Effects of Temperature on Phenotypic Expression Activity
5.1.04: Exercise 26 - Lab Wrap-Up
5.02: Exercise 27 - Effect of pH on Microbial Growth
5.2.01: pH and Microbial Growth
5.2.02: Assessing Effects of pH on Microbial Growth Activity
5.2.03: Exercise 27 - Lab Wrap-Up
5.03: Exercise 28 - Effect of Antiseptics and Disinfectants on Microbial Growth
5.3.01: Antiseptics and Disinfectants
5.3.02: Assessing the Effects of Antiseptics and Disinfectants on Microbial Growth Activity
5.3.03: Exercise 28 - Lab Wrap-Up
5.04: Exercise 29 - Antimicrobial Sensitivity Test (Kirby-Bauer Method)
5.4.01: Antimicrobial Susceptibility Test
5.4.02: Antimicrobial Sensitivity Test (Kirby-Bauer Method) Activity
5.4.03: Exercise 29 - Lab Wrap-Up
5.05: Section Five Review
5.5.01: Matching
5.5.02: Fill-In-The-Blank
5.5.03: True False
5.5.04: Multiple Choice Multiple Select
06: Section Six - Eukaryotic Pathogens (A Survey of Fungal Protozoan and Helminth Pathogens)
6.01: Exercise 30 - Fungal Pathogens
6.1.01: Fungi
6.1.02: Survey of Clinically Significant Yeasts
6.1.03: Survey of Clinically Significant Molds
6.1.04: Exercise 30 - Lab Wrap-Up
6.02: Exercise 31 - Protozoan Parasites
6.2.01: Protozoans
6.2.02: Subphylum Sarcodina (Amoeboids)
6.2.03: Subphylum Mastigophora (Flagellates)
6.2.04: Phylum Ciliata (Cilates)
6.2.05: Phylum Apicomplexa (Sporozoa)
6.2.06: Exercise 31 - Lab Wrap-Up
6.03: Exercise 32 - Helminth Pathogens
6.3.01: Helminths
6.3.02: Trematodes (Flukes)
6.3.03: Survey of Trematodes
6.3.04: Cestodes (Tapeworms)
6.3.05: Survey of Cestodes
6.3.06: Nematodes (Roundworms)
6.3.07: Survey of Nematodes
6.3.08: Exercise 32 - Lab Wrap-Up
6.04: Section Six Review
6.4.01: Matching
6.4.02: Fill-In-The-Blank
6.4.03: True-False
6.4.04: Multiple Choice Multiple Select
6.4.05: Identifying Parasites