Nguồn ngoài

Quantitative NMR

Quantitative nuclear magnetic resonance (Q-NMR) measurements are especially useful for analysis of complex samples where high specificity is needed or when a pure standard of the analyte is not available. This text provides a brief overview of basic NMR theory, a tutorial discussing the practical aspects of Q-NMR, a virtual experiment utilizing an NMR simulator, an exercise that makes use of real NMR data you can download and analyze, and a Q-NMR lab experiments.

Physical and Theoretical Chemistry Textbook Maps Chemistry

Tác giả: Cynthia K. Larive & Albert K. Korir
Nhà xuất bản: University of California, Riverside & Drury University
Nguồn: LibreTexts
Giấy phép: CC BY-NC-SA
Ngôn ngữ: EN
Danh mục: Khoa học cơ bản

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Mục lục· 39 mục

01: Basic NMR Theory
1.01: What is spin
1.02: How does absorption of energy generate an NMR spectrum
1.03: How does the population difference in NMR compare to the difference between electronic ground and excited states
1.04: What is chemical shift and how does it relate to resonance frequency
1.05: What is precession
1.06: How does precession generate the macroscopic magnetization (Mo)
1.07: How can the nuclear spins be manipulated to generate the NMR spectrum
1.08: What is the tip angle
1.09: What is the Free Induction Decay
1.10: How do T and T relaxation affect NMR spectra
1.11: Where should I look to learn more about NMR
02: Practical Aspects of Q-NMR
2.01: How do I choose a reference standard for my Q-NMR analysis
2.02: How is the internal standard used to quantify the concentration of my analyte
2.03: What sample considerations are important
2.04: How do I choose the right acquisition parameters for a quantitative NMR measurement
2.05: Effects of Tip Angle in Quantitative NMR Experiments
2.06: What data processing considerations are important for obtaining accurate and precise results
2.07: References
03: Virtual Experiment
3.01: Virtual Laboratory
04: Q-NMR Experiment
4.01: Prelab Exercises
4.02: Background
4.03: Dry Lab
4.04: Wet Lab
05: Q-NMR Applications
5.01: Q-NMR for purity determination of macrolide antibiotic reference standards- Comparison with the mass balance method
5.02: Determining Enantiomeric or Isomeric Purity of Active Pharmaceutical Ingredients
5.03: Q-NMR for Analysis and Characterization in Vaccine Preparations
5.04: Q-NMR-Based Metabonomics of Blood Samples
5.05: Q-NMR for Time Course Evolution of Malic and Lactic Acid
06: Instructor's Guide
6.01: Basic Theory Concept Questions
6.02: Answers to Questions in the Basic Theory section
6.03: Practical Aspects Concept Questions
6.04: Answers to Questions in the Practical Aspects section
6.05: Q-NMR Drylab